Return to study ST002115 main page

MB Sample ID: SA205793

Local Sample ID:HT1080_ML162_02
Subject ID:SU002231
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:HT1080

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN003513 AN003514
Analysis type MS MS
Chromatography type Reversed phase HILIC
Chromatography system Thermo Accela 1250 Thermo Accela 1250
Column Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) Waters XBridge BEH Amide (150 x 2.1mm,2.5um,100A)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Exactive Orbitrap Thermo Exactive Orbitrap
Ion Mode NEGATIVE NEGATIVE
Units Peak area (top) Peak area (top)

Chromatography:

Chromatography ID:CH002593
Chromatography Summary:The gradient was 0 min, 0% B; 2.5 min, 0% B; 5 min, 20% B; 7.5 min, 20% B; 13 min, 55% B; 15.5 min, 95% B; 18.5 min, 95% B; 19 min, 0% B; and 25 min, 0% B. Solvent A was 10 mM tributylamine and 15 mm acetic acid in water; Solvent B was methanol. The injection volume was 10 µL.
Instrument Name:Thermo Accela 1250
Column Name:Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um)
Column Temperature:40
Flow Rate:200
Solvent A:100% water; 15 mM acetic acid; 10 mM tributylamine
Solvent B:100% methanol
Chromatography Type:Reversed phase
  
Chromatography ID:CH002594
Chromatography Summary:The gradient was 0 min, 85% B; 2 min, 85% B; 3 min, 80% B; 5 min, 80% B; 6 min, 75% B; 7 min, 75% B; 8 min, 70% B; 9 min, 70% B; 10 min, 50% B; 12 min, 50% B; 13 min, 25% B; 16 min, 25% B; 18 min, 0% B; 23 min, 0% B; 24 min, 85% B; 30 min, 85% B. The injection volume was 5 µL.
Instrument Name:Thermo Accela 1250
Column Name:Waters XBridge BEH Amide (150 x 2.1mm,2.5um,100A)
Column Temperature:40
Flow Rate:150
Solvent A:95% water/5% acetonitrile; 20 mM ammonium acetate, pH 9.4
Solvent B:100% acetonitrile
Chromatography Type:HILIC
  logo