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MB Sample ID: SA206951
| Local Sample ID: | 190905_19_0009_UCLM_CCM_019 |
| Subject ID: | SU002243 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Cell Biosource Or Supplier: | CLS |
| Cell Strain Details: | In vitro spontaneously transformed keratinocytes from histologically normal skin |
| Cell Primary Immortalized: | HaCaT |
| Cell Passage Number: | Up to the 15th passage |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003532 |
|---|---|
| Analysis type | MS |
| Chromatography type | Ion pair |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Time of flight |
| MS instrument name | Waters LCT premier |
| Ion Mode | NEGATIVE |
| Units | Log2 (fold-change) |
Chromatography:
| Chromatography ID: | CH002609 |
| Chromatography Summary: | Chromatography was performed on a 1.0-mm internal diameter x 150 mm Acquity HSS T3 1.7 µm column (Waters Corp., Milford, MA) using an ACQUITY UPLC system (Waters Corp.). The column was maintained at 40 ºC. Samples (2µL) were injected onto the column at a flow rate of 100 µL/min, for a total run time of 25 min. The following linear elution gradient was used: 100% solvent A (10mM tributylamine + 15mM acetic acid + 0.2% methanol in water), to which solvent B (methanol) was added incrementally to reach a concentration of 4% B after 1.5 min, increasing to 20% B at 3 min, 25% B at 8 min, 50% B at 10 min, 55% at 15 min, and 100% B over the next 5 min, and returning to the initial composition over the final 5 min. |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | Ion pair |
