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MB Sample ID: SA210174

Local Sample ID:F1433
Subject ID:SU002277
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:E18.5
Gender:Male and female
Animal Housing:Vivarium University of Colorado Anschutz Medical Campus
Animal Light Cycle:12/12
Animal Feed:CH; D12450B; Research Diets, New Brunswick, NJ, USA or HFD; D12451; Research Diets
Animal Water:Water or treated with 1.25 mg/L PQQ

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Combined analysis:

Analysis ID AN003586
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units Peak area

Chromatography:

Chromatography ID:CH002650
Chromatography Summary:The analytical platform employed a Vanquish UHPLC system (Thermo Fisher Scientific, San Jose, CA, USA) coupled online to a Q Exactive mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). Lipidomics analyses were performed as described in previous method papers and application notes. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640979/, https://www.haematologica.org/article/view/9990, https://pubmed.ncbi.nlm.nih.gov/31119660/
Methods Filename:Fetal_and_weanling_lipid_protocols.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
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