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MB Sample ID: SA211932
Local Sample ID: | QC-10 |
Subject ID: | SU002303 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Combined analysis:
Analysis ID | AN003626 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Normalized Peak Area |
Chromatography:
Chromatography ID: | CH002681 |
Chromatography Summary: | Hydrophilic interaction liquid chromatography (HILIC) was performed at 0.2 ml/min on a ZIC-pHILIC column (2.1 mm × 150 mm, EMD Millipore) at 45 °C. Solvent A was 20 mM ammonium carbonate, 0.1% ammonium hydroxide, pH 9.2, and solvent B was acetonitrile. The gradient was 85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min. The autosampler was held at 4 °C. For each analysis, 4 µl of sample was injected. |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
Flow Gradient: | 85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min. |
Solvent A: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide, pH 9.6 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |