Return to study ST002233 main page
MB Sample ID: SA212716
| Local Sample ID: | batch16_MT_20210810_001C_20210809143500 |
| Subject ID: | SU002319 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003643 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Scientific Vanquish Horizon UHPLC system |
| Column | HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid |
| Ion Mode | POSITIVE |
| Units | m/z values |
Chromatography:
| Chromatography ID: | CH002697 |
| Chromatography Summary: | Mobile phase: 10 mM ammonium acetate in 95% acetonitrile containing 0.1% acetic acid as mobile phase A and 10 mM ammonium acetate in 50% acetonitrile containing 0.1% acetic acid as mobile phase B were used for HILIC method. Mobile phase gradient: For HILIC acquisition, following gradient was applied at a flow rate of 0.55 ml/min: 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column.For RP column, following gradient was applied at a flow rate of 0.4 ml/min: 0-0.1 min: 0% B, 0.10-1.9 min: 60% B, 1.9-5.0 min: 98% B, 5.00-5.10 min: 0% B and 4.9 min cleaning and column equilibration. |
| Instrument Name: | Thermo Scientific Vanquish Horizon UHPLC system |
| Column Name: | HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column. |
| Flow Rate: | 0.55 ml/min |
| Solvent A: | 95% acetonitrile/5% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
