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MB Sample ID: SA217682
| Local Sample ID: | H_N_054 |
| Subject ID: | SU002355 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 8.7-18.6 (year) |
| Gender: | Male and female |
| Human Ethnicity: | Korean |
| Human Inclusion Criteria: | We recruited children and adolescent participants who visited the pediatric departments of the Hallym University Sacred Heart Hospital and Seoul National University Children’s Hospital from January 2019 to May 2020, after obtaining informed consent from the children and their parents. |
| Human Exclusion Criteria: | We excluded participants who were taking alcohol or medications known to affect the results of liver function tests. Participants with viral hepatitis, such as hepatitis A, B, or C, or with Epstein–Barr virus, Wilson’s disease, autoimmune hepatitis, or muscular disease were also excluded. |
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Combined analysis:
| Analysis ID | AN003706 | AN003707 | AN003708 | AN003709 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | NA | NA |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | μM | μM | μM | μM |
Chromatography:
| Chromatography ID: | CH002745 |
| Chromatography Summary: | LC1 method for analysis of amino acids and biogenic amines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) |
| Column Temperature: | 50 |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002746 |
| Chromatography Summary: | LC2 method for analysis of isoleucine For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) |
| Column Temperature: | 50 |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002747 |
| Chromatography Summary: | FIA1 method for analysis of acylcarnitines and lipids For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | NA |
| Solvent A: | 290mL Methanol + 1 ampule FIA mobile phase additive |
| Chromatography Type: | None (Direct infusion) |
| Chromatography ID: | CH002748 |
| Chromatography Summary: | FIA2 method for analysis of acylcarnitines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | NA |
| Solvent A: | 290mL Methanol + 1 ampule FIA mobile phase additive |
| Chromatography Type: | None (Direct infusion) |
