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MB Sample ID: SA217798
| Local Sample ID: | 2110-C-R2 |
| Subject ID: | SU002358 |
| Subject Type: | Plant |
| Subject Species: | Hordeum vulgare |
| Taxonomy ID: | 4513 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003714 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | nmoles/mg DW and arbitrary/mg DW |
Chromatography:
| Chromatography ID: | CH002751 |
| Chromatography Summary: | The instrument was an Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass spectrometer. The column was an Rxi-5SilMS from Restek (30 m with 10 m integraguard column). The liner (Restek # 20994) was changed before each derivatization series. Oven temperature ramp was 70 °C for 7 min then 10 °C/min to 330 °C for 5 min (run length 38 min). Helium constant flow was 0.7 mL/min. Temperatures were the following: injector: 250°C, transfer line: 290°C, source: 250 °C and quadripole 150 °C. 5 scans per second were acquired spanning a 50 to 600 Da range. Instrument was tuned with PFTBA with the 69 m/z and 219 m/z of equal intensities. 5 scans per second were acquired. The split mode conditions were: 70°C for 2 min then 30°C per min to 330 °C for 5 min. Helium constant flow 1 mL/min. |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
| Flow Rate: | 0.7 ml/min |
| Injection Temperature: | 250°C |
| Chromatography Type: | GC |
