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MB Sample ID: SA217807
Local Sample ID: | 4710-C-R3 |
Subject ID: | SU002358 |
Subject Type: | Plant |
Subject Species: | Hordeum vulgare |
Taxonomy ID: | 4513 |
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Combined analysis:
Analysis ID | AN003714 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975C |
Ion Mode | POSITIVE |
Units | nmoles/mg DW and arbitrary/mg DW |
Chromatography:
Chromatography ID: | CH002751 |
Chromatography Summary: | The instrument was an Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass spectrometer. The column was an Rxi-5SilMS from Restek (30 m with 10 m integraguard column). The liner (Restek # 20994) was changed before each derivatization series. Oven temperature ramp was 70 °C for 7 min then 10 °C/min to 330 °C for 5 min (run length 38 min). Helium constant flow was 0.7 mL/min. Temperatures were the following: injector: 250°C, transfer line: 290°C, source: 250 °C and quadripole 150 °C. 5 scans per second were acquired spanning a 50 to 600 Da range. Instrument was tuned with PFTBA with the 69 m/z and 219 m/z of equal intensities. 5 scans per second were acquired. The split mode conditions were: 70°C for 2 min then 30°C per min to 330 °C for 5 min. Helium constant flow 1 mL/min. |
Instrument Name: | Agilent 7890A |
Column Name: | Restek Rxi-5Sil (30m x 0.25mm,0.25m) with 10m precolumn |
Flow Rate: | 0.7 ml/min |
Injection Temperature: | 250°C |
Chromatography Type: | GC |