Return to study ST002297 main page
MB Sample ID: SA220909
| Local Sample ID: | L37_V6 |
| Subject ID: | SU002383 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003752 | AN003753 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Waters Atlantis dC18 (100 x 3mm,3um) | Waters Atlantis dC18 (100 x 3mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area (pareto-scaled, log2-transformed, 24h creatinine normalised) | peak area (pareto-scaled, log2-transformed, 24h creatinine normalised) |
Chromatography:
| Chromatography ID: | CH002777 |
| Chromatography Summary: | Analysis of serum and urine samples was performed using a published LC-QTOF-MS acquisition method, which employed a 1290 Infinity II HPLC coupled to a 6550 QTOF-MS equipped with dual AJS electrospray ionisation source (Agilent, Cheadle, UK). Data acquisition parameters are detailed in brief below and in full in Supplementary Materials. Reversed-phase LC was performed on an Atlantis dC18 column (3×100 mm, 3 μm, Waters, Manchester, UK) maintained at 60 °C. Mobile phase composition was (A) water and (B) methanol, both with 5 mmol/L ammonium formate and 0.1 % formic acid. The elution gradient began at 5 % B 0–1 min and increased linearly to 100 % B by 12 min, held at 100 % B until 14 min, then at 5 % B for a further 5 min. MS data acquisition was performed in positive and negative ionisation polarity with mass range 50–1700 in 2 GHz mode with acquisition rate at 3 spectra/second. Sample injection volume was 1 and 2 µL in negative and positive polarities, respectively. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Waters Atlantis dC18 (100 x 3mm,3um) |
| Column Temperature: | 60 |
| Flow Gradient: | The elution gradient began at 5 % B 0–1 min and increased linearly to 100 % B by 12 min, held at 100 % B until 14 min, then at 5 % B for a further 5 min. |
| Solvent A: | 100% water; 0.1% formic acid; 5mM ammonium formate |
| Solvent B: | 100% methanol; 0.1% formic acid; 5mM ammonium formate |
| Chromatography Type: | Reversed phase |
