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MB Sample ID: SA241941

Local Sample ID:F2-27-09
Subject ID:SU002502
Subject Type:Cultured cells
Subject Species:Homo sapiens

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Combined analysis:

Analysis ID AN003933 AN003934
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH002912
Chromatography Summary:17 minute negative
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:45
Flow Gradient:0-1 min 25% B and 0.3 ml/min; 1-2 min 25-50% B and 0.3 mL/min; 2–8 min 50–90% B and 0.3 mL/min; 8–10 min 90–99% B and 0.3 mL/min; 10–14 min hold at 99% B and 0.3 mL/min; 14–14.1 min 99–25% B and 0.3 mL/min; 14.1–16.9 min hold at 25% B and 0.4 mL/min; 16.9–17 min hold at 25% B and resume flow of 0.3 mL/min
Flow Rate:varies, see solvent gradient
Solvent A:25% acetonitrile; 5 mM ammonium acetate
Solvent B:50% isopropanol/45% acetonitrile/5% water; 5 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH002913
Chromatography Summary:17 minute positive
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Column Temperature:45
Flow Gradient:0–10 min 30–100% B and 0.325 mL/min; 10–12 min 100% B and 0.325 mL/min; 12–12.5 min 100–30% B and 0.4 mL/min; 12.5–14.9 min 30% B and 0.4 mL/min; 14.9–15 min 30% B and 0.325 mL/min.
Flow Rate:varies, see solvent gradient
Solvent A:25% acetonitrile/75% water; 5 mM ammonium acetate
Solvent B:90% isopropanol/10% acetonitrile; 5 mM ammonium acetate
Chromatography Type:Reversed phase
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