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MB Sample ID: SA247791
| Local Sample ID: | 36-week db/db+aav9-RDH10-2 |
| Subject ID: | SU002564 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | db/m; db/db; db/db+retinol; db/db+retinoic acid; db/db+AAV9-RDH10; RDH10-cKO; RDH10fl/fl |
| Age Or Age Range: | 32-week; 36-week; 21-week |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004041 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Sciex Triple Quad TM 4500MD MS |
| Column | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH002989 |
| Instrument Name: | Sciex Triple Quad TM 4500MD MS |
| Column Name: | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | initial conditions 75% B; from 0 to 3.5min linear increase to 95% B; between 3.5 and 4.5min 95% B was retained; at 4.6 min back to initial conditions with 75% B; finally, 75% B was held from 4.6 to 5.9min |
| Flow Rate: | 0.2ml/min |
| Solvent A: | 40% acetonitrile/30% methanol/30% water; 0.1 % formic acid |
| Solvent B: | 55% acetonitrile/30% methanol/15% water; 0.1 % formic acid |
| Chromatography Type: | Reversed phase |
