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MB Sample ID: SA249126
| Local Sample ID: | FB06135270 |
| Subject ID: | SU002586 |
| Subject Type: | Plant |
| Subject Species: | Malus domestica/Musa acuminata/Solanum lycopersicum/Lactuca sativa/Fragaria x ananassa/Daucus carota/Prunus persica/Allium cepa/Spinacia oleracea/Capsicum annuum/Zea mays/Allium sativum/Ocimum basilicum/Solanum tuberosum/Glycine max/Phaseolus vulgaris/Olea europaea/Cicer arietinum/Beta vulgaris/Pyrus communis |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004081 | AN004082 | AN004083 | AN004084 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus | Thermo Q Exactive Focus | Thermo Q Exactive Focus | Thermo Q Exactive Focus |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003023 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | 0-1 min 99% (A), 1-13min 99%-65% (A), 13-14.5min 65%-30% (A), 14.5-15.5min 30%-1% (A), 15.5-17min 1% (A), 17-17.5min 1%-99% (A) |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 100% water; 0.05% perfluoropentanoic acid; 0.1% formic acid |
| Solvent B: | 95% methanol/5% water; 0.05% perfluoropentanoic acid; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003024 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | 0-1min 45% (A), 1-4min 45%-35% (A), 4-12min 25%-11% (A), 12-15min 11%-1% (A) |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 95% methanol/5% water; 6.5mM Ammonium Bicarbonate at pH 8 |
| Solvent B: | 70% acetonitrile/30% water; 6.5mM Ammonium Bicarbonate at pH 8 |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003025 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40C |
| Flow Gradient: | 0-4min 100% (A), 4-6min 100%-50% (A), 6-11min 50%-20% (A), 11-12min 20%-0% (A), 12-14min 0% (A) |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 10mM Ammonium Formate, pH 10.8 |
| Solvent B: | 100% acetonitrile; 10mM Ammonium Formate, pH 10.8 |
| Chromatography Type: | HILIC |
