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MB Sample ID: SA249253
| Local Sample ID: | sample_0043 |
| Subject ID: | SU002588 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN004092 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 7890B |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 7200 QTOF |
| Ion Mode | NEGATIVE |
| Units | Raw output from software |
Chromatography:
| Chromatography ID: | CH003030 |
| Chromatography Summary: | The plate was preconditioned with 450 μL acetonitrile before the addition of 100 μL of sample and 10 μL of PFAS and BA internal standard mixture (200 ng/mL and 1000 ng/mL respectively). Thereafter, 450 μL of acetonitrile containing 1% formic acid were added to each well and the samples extracted using a 10” vacuum manifold. The eluate evaporated to dryness under nitrogen gas flow and reconstituted to 80 μL of MeOH/2 mM aqueous NH4AC. Chromatographic separation was carried out using an Acquity UPLC BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm particle size), fitted with a C18 precolumn (Waters Corporation, Wexford, Ireland). Mobile phase A consisted of H2O:MeOH (v/v 70:30) and mobile phase B of MeOH with both phases containing 2mM ammonium acetate as an ionization agent. The flow rate was set at 0.4 mLmin-1 with the elution gradient as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%; 1.5-4.5 min, mobile phase B increased to 70%; 4.5-7.5 min, mobile phase B increased to 100% and held for 5.5 min. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total run time per sample was 18 min. The dual ESI ionization source was settings were as follows: capillary voltage was 4.5 kV, nozzle voltage 1500 V, N2 pressure in the nebulized was 21 psi and the N2 flow rate and temperature as sheath gas was 11 L min-1 and 379 °C, respectively. |
| Instrument Name: | Agilent 7890B |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 70 |
| Flow Gradient: | The elution gradient was as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%; 1.5-4.5 min, mobile phase B increased to 70%; 4.5-7.5 min, mobile phase B increased to 100% and held for 5.5 min. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total run time per sample was 18 min. |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 70% water/30% methanol |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
