Return to study ST002503 main page
MB Sample ID: SA251331
| Local Sample ID: | KO1n_13_FA |
| Subject ID: | SU002600 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004112 | AN004113 | AN004114 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Thermo Vanquish | Thermo Vanquish |
| Column | Waters CORTECS UPLC C18 (150 x 2.1mm,1.6um) | Waters CORTECS HILIC (100 x 2.1mm,1.6um) | Waters CORTECS HILIC (100 x 2.1mm,1.6um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | Orbitrap | Orbitrap |
| MS instrument name | Agilent 6545 QTOF | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH003047 |
| Methods Filename: | LC_Method.pdf |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Waters CORTECS UPLC C18 (150 x 2.1mm,1.6um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0-2 min, 30% B; 17 min, 75% B; 20 min, 85% B; 23-26 min, 100% B; 26 min, 30% B followed by a re-equilibration phase of 5 min. |
| Flow Rate: | .25ml/min |
| Solvent A: | A: 60% acetonitrile, 40% water, 0.1% formic acid, 10 mM ammonium formate, 2.5 mM medronic acid |
| Solvent B: | 90% 2-propanol, 10% acetonitrile, 0.1% formic acid, 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003048 |
| Methods Filename: | LC_Method.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters CORTECS HILIC (100 x 2.1mm,1.6um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 |
| Flow Rate: | .25ml/min |
| Solvent A: | A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 2.5 μM medronic acid |
| Solvent B: | B: 95% acetonitrile, 5% water, 2.5 μM medronic acid |
| Chromatography Type: | HILIC |
