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MB Sample ID: SA253164

Local Sample ID:P1-E01
Subject ID:SU002615
Subject Type:Bacteria
Subject Species:Eggerthella lenta
Taxonomy ID:84112
Genotype Strain:DSM 2243, Valencia, AB8n2

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Combined analysis:

Analysis ID AN004142
Analysis type MS
Chromatography type Unspecified
Chromatography system Agilent 1290 Infinity II
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6545 QTOF
Ion Mode NEGATIVE
Units mM

Chromatography:

Chromatography ID:CH003067
Chromatography Summary:Samples were injected via refrigerated autosampler into mobile phase and chromatographically separated by an Agilent 1290 Infinity II UPLC and detected using an Agilent 6545XT Q-TOF (quadrupole time of flight) mass spectrometer equipped with a dual jet stream electrospray ionization source, operating under extended dynamic range (1,700 m/z). Chromatographic separation was performed using an ACQUITY Bridged Ethylene Hybrid (BEH) C18 column 2.1 x 100 mm, 1.7-micron particle size, (Waters Corp. Milford, MA), using chromatographic conditions published elsewhere (Supplementary Table S26 of https://doi.org/10.1038/s41564-022-01109-9).
Instrument Name:Agilent 1290 Infinity II
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:See
Flow Rate:300 μL per minute
Solvent A:0.1% formic acid in water
Solvent B:0.1% formic acid in methanol
Chromatography Type:Unspecified
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