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MB Sample ID: SA254928

Local Sample ID:1-1_nontumor
Subject ID:SU002634
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004169
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column SeQuant ZIC-HILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap ID-X tribrid
Ion Mode UNSPECIFIED
Units Peak areas

Chromatography:

Chromatography ID:CH003087
Chromatography Summary:Metabolites were separated via hydrophilic interaction liquid chromatography (HILIC) by using a SeQuant ZIC-pHILIC column (100 x 2.1 mm, 5 µm, polymer, Merck-Millipore) with a ZIC-pHILIC guard column (20 mm x 2.1 mm, 5 µm). The column compartment temperature was 40 °C and the flow rate was set to 250 µL min-1. The mobile phases consisted of A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 5 µM medronic acid; and B: 95% acetonitrile, 5% water. The following linear gradient was applied: 0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 min at 400 µL min-1 and 2 min at 250 µL min-1. The samples were kept at 6 °C in the autosampler. The injection volume was 5 µL.
Instrument Name:Thermo Vanquish
Column Name:SeQuant ZIC-HILIC (150 x 2.1mm,5um)
Column Temperature:40 °C
Flow Gradient:0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 min at 400 µL min-1 and 2 min at 250 µL min-1
Flow Rate:250 µL min-1
Solvent A:95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 5 µM medronic acid
Solvent B:95% acetonitrile, 5% water
Chromatography Type:HILIC
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