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MB Sample ID: SA254943
Local Sample ID: | 4-2_serum |
Subject ID: | SU002634 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004169 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Orbitrap ID-X tribrid |
Ion Mode | UNSPECIFIED |
Units | Peak areas |
Chromatography:
Chromatography ID: | CH003087 |
Chromatography Summary: | Metabolites were separated via hydrophilic interaction liquid chromatography (HILIC) by using a SeQuant ZIC-pHILIC column (100 x 2.1 mm, 5 µm, polymer, Merck-Millipore) with a ZIC-pHILIC guard column (20 mm x 2.1 mm, 5 µm). The column compartment temperature was 40 °C and the flow rate was set to 250 µL min-1. The mobile phases consisted of A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 5 µM medronic acid; and B: 95% acetonitrile, 5% water. The following linear gradient was applied: 0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 min at 400 µL min-1 and 2 min at 250 µL min-1. The samples were kept at 6 °C in the autosampler. The injection volume was 5 µL. |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
Column Temperature: | 40 °C |
Flow Gradient: | 0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 min at 400 µL min-1 and 2 min at 250 µL min-1 |
Flow Rate: | 250 µL min-1 |
Solvent A: | 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 5 µM medronic acid |
Solvent B: | 95% acetonitrile, 5% water |
Chromatography Type: | HILIC |