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MB Sample ID: SA256113
| Local Sample ID: | D_Serum_4 |
| Subject ID: | SU002650 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004198 | AN004199 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nM | nM |
Chromatography:
| Chromatography ID: | CH003111 |
| Chromatography Summary: | LC-MS/MS (MRM) for phospholipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | isocratic condition of 60% B |
| Flow Rate: | 400 µL/min |
| Solvent A: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(900/50/50) |
| Solvent B: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(940/50/10) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003112 |
| Chromatography Summary: | LC-MS/MS (MRM) for sphingolipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 50% A for 0 min, 50% A for 5 min, 50 to 30% A for 3 min, 30% A for 7 min, 30 to 10% A for 7 min, 10% A for 3 min, 10 to 50% A for 0.1 min, and 50% A for 4.9 min |
| Flow Rate: | 200 µL/min |
| Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran(500/200/300) |
| Solvent B: | 5 mM ammonium formate/MeOH/tetrahydrofuran(100/200/700) |
| Chromatography Type: | Reversed phase |
