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MB Sample ID: SA258359
| Local Sample ID: | PTEN WT Rep1 |
| Subject ID: | SU002673 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | embryonic fibroblasts |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004237 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | APCI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | peak area intensity |
Chromatography:
| Chromatography ID: | CH003144 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| Column Temperature: | 45 |
| Flow Gradient: | 85% buffer B to 30% buffer B from 0 - 3 minutes, then 30% buffer B to 3% buffer B from minute 3 to 12, then 2% buffer B held from minute 12 to 15, followed by 2% buffer B to 85% buffer B from minute 15 to 16, then 85% buffer B held from minute 16-23 in order to re-equilibrate the column. |
| Flow Rate: | 400uL/minute |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide; 20 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
