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MB Sample ID: SA258890
| Local Sample ID: | 90254013110 |
| Subject ID: | SU002681 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004250 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo TSQ Quantiva |
| Ion Mode | POSITIVE |
| Units | Micromolar |
Chromatography:
| Chromatography ID: | CH003156 |
| Chromatography Summary: | Analytes were chromatographically separated with 40 minutes LC gradient |
| Methods Filename: | pass1b_amines_methods.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
| Column Temperature: | 43C |
| Flow Gradient: | t=0 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=1 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=2 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=5.5 min: 98.1%/1.9% (%A/%B) @ 0.4 ml/min | t=6.5 min: 98%/2% (%A/%B) @ 0.4 ml/min | t=10 min: 97.5%/2.4% (%A/%B) @ 0.4 ml/min | t=12 min: 96%/4% (%A/%B) @ 0.4 ml/min | t=20 min: 88%/12% (%A/%B) @ 0.4 ml/min | t=27 min: 86.5%/13.5% (%A/%B) @ 0.4 ml/min | t=31 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=32 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35.5 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=39 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min |
| Flow Rate: | 0.4 ml/min; 0.5 ml/min |
| Solvent A: | 1% acetonitrile/99% water; 0.1% formic acid |
| Solvent B: | 1% water, 99% Acetonitrile |
| Chromatography Type: | Reversed phase |
