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MB Sample ID: SA271300

Local Sample ID:R Control 2A-01_14_1_3231
Subject ID:SU002808
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN004383
Analysis type MS
Chromatography type Reversed phase
Chromatography system Bruker timsTOF
Column Hamilton Intensity Solo 2 C18(100 x 2.1 mm, 1.8 um)
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker timsTOF
Ion Mode POSITIVE
Units AU

Chromatography:

Chromatography ID:CH003288
Chromatography Summary:Then, liquid chromatography separation was done using Intensity Solo 1.8 C18-2 column (100×2.1 mm, 1.8 μm) from Bruker Daltonik GmbH (Bremen, Germany). The column temperature was kept at 35 ℃ and the flow rate was constant at 0.3 ml/min. The mobile phase composed of 0.1% FA in HPLC grade water (solvent A) and 0.1% FA in ACN (solvent B).
Instrument Name:Bruker timsTOF
Column Name:Hamilton Intensity Solo 2 C18(100 x 2.1 mm, 1.8 um)
Column Temperature:35
Flow Gradient:1% B was held for 2 min, ramping to 99% B over 15 min, and held at 99% B for 3 min before re-equilibrating to 1% B for 10 min
Flow Rate:250 uL/min
Solvent A:Water (0.1% Formic Acid)
Solvent B:ACN (0.1% Formic Acid)
Chromatography Type:Reversed phase
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