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MB Sample ID: SA272321

Local Sample ID:RF39
Subject ID:SU002813
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004390
Analysis type MS
Chromatography type HILIC
Chromatography system Vanquish™ Flex UHPLC
Column Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units a.u.

Chromatography:

Chromatography ID:CH003293
Chromatography Summary:ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA).
Instrument Name:Vanquish™ Flex UHPLC
Column Name:Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Column Temperature:25
Flow Gradient:linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B
Flow Rate:0.150µl/min
Solvent A:100% acetonitrile
Solvent B:100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide
Chromatography Type:HILIC
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