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MB Sample ID: SA274730
| Local Sample ID: | 52 |
| Subject ID: | SU002835 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004423 | AN004424 | AN004425 | AN004426 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Ion exchange | Reversed phase | Reversed phase | Normal phase |
| Chromatography system | Thermo Dionex ICS-5000+ | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera UC |
| Column | Dionex IonPac AS11-HC (250 x 2mm,4um) | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) | GL Sciences Inert Sustain C18 (150 × 2.1mm, 3um) | Waters ACQUITY UPC2 Torus DEA (100 x 3.0mm, 1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Triple quadrupole |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Shimadzu LCMS-8060 |
| Ion Mode | UNSPECIFIED | UNSPECIFIED | POSITIVE | UNSPECIFIED |
| Units | relative peak area | relative peak area | concentration (pmol/mg-protein) | relative peak area |
Chromatography:
| Chromatography ID: | CH003322 |
| Chromatography Summary: | Anionic polar metabolites (i.e., organic acids, sugar phosphates, nucleotides, etc.) were analyzed via IC/HRMS/MS. |
| Instrument Name: | Thermo Dionex ICS-5000+ |
| Column Name: | Dionex IonPac AS11-HC (250 x 2mm,4um) |
| Column Temperature: | 30℃ |
| Flow Gradient: | 10-100%A, 0-24.0 min, 100%A, 24.0-27.0 min, 100-10%A, 27.0-27.1 min, 10%A, 27.1-35.0 min |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100 mM KOH in H2O |
| Solvent B: | 1 mM ammonium acetate in MeOH |
| Chromatography Type: | Ion exchange |
| Chromatography ID: | CH003323 |
| Chromatography Summary: | Cationic polar metabolites (i.e., amino acids, bases, nucleosides, etc) were analyzed via PFPP-LC/HRMS/MS. |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0%B, 0-5.0 min, 0-40%B, 5.0-15.0 min, 40-100%B, 15.0-15.1 min, 100%B, 15.1-18.0 min, 100-0%B, 18.0-18.1 min, 0%B, 18.1-25.0 min |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 0.1% formic acid in H2O |
| Solvent B: | ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003324 |
| Chromatography Summary: | Acyl-CoA and acyl-carnitine were analyzed via C18-LC/HRMS/MS. |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | GL Sciences Inert Sustain C18 (150 × 2.1mm, 3um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0-13.0 min, 2-95%B, 13.0-20.0 min, 95%B, 20.0-20.1 min, 95-2%B, 20.1-25.0 min, 2%B |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 5 mM ammonium acetate in H2O |
| Solvent B: | ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003325 |
| Chromatography Summary: | Fatty acids (FA), diacylglycerols (DG), and triacylglycerols (TG) were analyzed via Normal phase SFC/MS/MS. |
| Instrument Name: | Shimadzu Nexera UC |
| Column Name: | Waters ACQUITY UPC2 Torus DEA (100 x 3.0mm, 1.7um) |
| Column Temperature: | 50℃ |
| Flow Gradient: | 0-1.0 min, 1%B, 1.0-24.0 min, 1-75%B, 24.0-26.0 min, 75%B, 26.0-26.1 min, 75-1%B, 26.1-30.0 min, 1%B |
| Flow Rate: | 1.0 mL/min |
| Solvent A: | CO2 |
| Solvent B: | 0.1% (w/v) ammonium acetate in MeOH/H2O (95/5) |
| Chromatography Type: | Normal phase |
| Solvent C: | 0.1% (w/v) ammonium acetate in MeOH/H2O (95/5) |
