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MB Sample ID: SA274767
Local Sample ID: | Solid Media 4-02-4797 |
Subject ID: | SU002836 |
Subject Type: | Yeast |
Subject Species: | Candida albicans |
Taxonomy ID: | 5476 |
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Combined analysis:
Analysis ID | AN004427 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Bruker timsTOF |
Column | Hamilton Intensity Solo 2 C18 |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker timsTOF |
Ion Mode | POSITIVE |
Units | AU |
Chromatography:
Chromatography ID: | CH003326 |
Chromatography Summary: | 10 µL aliquot of the sample was injected and the separation was performed on a Hamilton® Intensity Solo C18 column (2.1 × 100 mm, 1.8 µm) (Bruker Daltonik) in a column oven temperature set at 35 ◦C , using solvent A (0.1% formic acid in deionized Water) and solvent B (0.1% formic acid in acetonitrile) with the following gradient elution mode: 0 to 2 min, 1% B; 2 to 17 min, 1–99% B; 17 to 20 min, 99% B; 20 to 20.1 min, 99–1% B; 20.1 to 30 min, 1% B. The flow rate was 0.25 mL/min from 0 to 20 min, 0.35 mL/min from 20 min to 28.3 min, and 0.25 mL/min from 28.3 to 30 min. |
Instrument Name: | Bruker timsTOF |
Column Name: | Hamilton Intensity Solo 2 C18 |
Column Temperature: | 35 |
Flow Gradient: | gradient elution mode: 0 to 2 min, 1% B; 2 to 17 min, 1–99% B; 17 to 20 min, 99% B; 20 to 20.1 min, 99–1% B; 20.1 to 30 min, 1% B |
Flow Rate: | 250 uL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |