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MB Sample ID: SA288685
Local Sample ID: | RJ_3CC1 |
Subject ID: | SU002843 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004438 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | peak area |
Chromatography:
Chromatography ID: | CH003334 |
Chromatography Summary: | HILIC chromatographic separation of metabolites was achieved using a Millipore Sequant ZIC-pHILIC analytical column (5 µm, 2.1 × 150 mm) equipped with a 2.1 × 20 mm guard column (both 5 mm particle size) with a binary solvent system. Solvent A was 20 mM ammonium carbonate, 0.05% ammonium hydroxide; Solvent B was acetonitrile. The column oven and autosampler tray were kept at 40 °C and 4 °C, respectively. The chromatographic gradient was run at a flow rate of 0.200 mL/min as follows: 0–2 min: 80% B; 2-17 min: linear gradient from 80% B to 20% B; 17-17.1 min: linear gradient from 20% B to 80% B; 17.1-22.5 min: hold at 80% B. Samples were randomized and analysed with LC–MS in a blinded manner with an injection volume was 5 µl. Pooled samples were generated from an equal mixture of all individual samples and analysed interspersed, at regular intervals, within the sample sequence as a quality control. Each sample was analysed with three analytical replicates. |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 40 |
Flow Gradient: | 0–2 min: 80% B; 2-17 min: linear gradient from 80% B to 20% B; 17-17.1 min: linear gradient from 20% B to 80% B; 17.1-22.5 min: hold at 80% B |
Flow Rate: | 0.200 mL/min |
Solvent A: | 20 mM ammonium carbonate, 0.05% ammonium hydroxide |
Solvent B: | acetonitrile |
Chromatography Type: | HILIC |