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MB Sample ID: SA289303
| Local Sample ID: | VIAH002_BK_1_Pos_QE1_Lipids_006 |
| Subject ID: | SU002854 |
| Subject Type: | Cultured cells |
| Subject Species: | Rickettsia parkeri; Homo sapiens |
| Taxonomy ID: | 35792; 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004454 | AN004455 | AN004456 | AN004457 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | counts, height | counts, height | counts, height | counts, height |
Chromatography:
| Chromatography ID: | CH003345 |
| Chromatography Summary: | HILIC |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45C |
| Flow Gradient: | Gradient elution was performed from 100% (B) at 0–2 min to 70% (B) at 7.7 min, 40% (B) at 9.5 min, 30% (B) at 10.25 min, 100% (B) at 12.75 min, isocratic until 16.75 min with a column flow of |
| Flow Rate: | 0.4 mL/min. |
| Solvent A: | 100% water; 10mM ammonium formate; 0.125% formic acid |
| Solvent B: | 95% acetonitrile; 10mM ammonium formate; 0.125% formic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003346 |
| Chromatography Summary: | Lipids (ammonium acetate replaces ammonium formate in neg mode analysis |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | Gradient elution was performed from 15% (B) at 0 min to 30% (B) at 2 min, 48% (B) at 2.5 min, 82% (B) at 11 min, 99% (B) at 11.5 min, isocratic until 12 and resetting to initial conditions at 12.1 min through the end of the run at 15 min. |
| Flow Rate: | is 0.6 mL/min. |
| Solvent A: | 60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
