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MB Sample ID: SA290500
| Local Sample ID: | 20211007_Cowley3_Plasma_18_55-429-LS-A_C18pos_rep |
| Subject ID: | SU002865 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
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Combined analysis:
| Analysis ID | AN004475 | AN004476 | AN004477 | AN004478 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Area | Area | Area | Area |
Chromatography:
| Chromatography ID: | CH003360 |
| Chromatography Summary: | This chromatography method was utilized for all C18 positive polarity runs in this study. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) |
| Column Temperature: | 25C |
| Flow Gradient: | 0-1 minutes at 98% A1/2% B1, 1-13 minutes from 98% A1/2% B1 to 10% A1/90% B1, 13-15 minutes at 10% A1/90% B1, 15-16 minutes from 10% A1/90% B1 to 98% A1/2% B1, and was re-equilibrated from 16-25 minutes at 98% A1/2% B1 |
| Flow Rate: | 0.1 mL/minute |
| Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
| Solvent A: | 100% water, 0.2% acetic acid |
| Solvent B: | 100% acetonitrile, 0.2% acetic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003361 |
| Chromatography Summary: | This chromatography method was utilized for all C18 negative polarity runs in this study. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
| Column Temperature: | 25C |
| Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
| Flow Rate: | 0.1 mL/minute |
| Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
| Solvent A: | 100% water, 0.2% acetic acid |
| Solvent B: | 100% acetonitrile, 0.2% acetic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003362 |
| Chromatography Summary: | This chromatography method was utilized for all HILIC positive polarity runs in this study. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
| Column Temperature: | 25C |
| Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
| Flow Rate: | 0.1 mL/minute |
| Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
| Solvent A: | 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent A2) |
| Solvent B: | 90% ACN with 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent B2) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003363 |
| Chromatography Summary: | This chromatography method was utilized for all HILIC negative polarity runs in this study. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
| Column Temperature: | 25C |
| Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
| Flow Rate: | 0.1 mL/minute |
| Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
| Solvent A: | 10 mM ammonium acetate in H2O, pH 9.0 with 0.1% AffinityLab Deactivator Inhibitor (Agilent, #5191-3940; Solvent A3) |
| Solvent B: | 85% ACN with 10 mM ammonium acetate in H2O with 0.1% AffinityLab Deactivator Inhibitor (Solvent B3) |
| Chromatography Type: | HILIC |
