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MB Sample ID: SA301652
Local Sample ID: | 330P |
Subject ID: | SU002918 |
Subject Type: | Cultured cells |
Subject Species: | Lentilactobacillus kefiri |
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Combined analysis:
Analysis ID | AN004571 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5977A |
Ion Mode | POSITIVE |
Units | normalized intensity |
Chromatography:
Chromatography ID: | CH003436 |
Chromatography Summary: | The GC-MS analysis was carried out with an Agilent GC (Model 7890B) and MS Quadrupole (Model 5977A) (Agilent Technologies). The liquid injection was done using a PAL System RSI 85 (PAL, Lake Elmo, MN, USA). The injector temperature was 260°C; the MS transfer line was 230°C. Metabolites were separated on a 5% phenyl 95% dimethylarylene siloxane HP-5MS 30 m, 0.25 mm, 0.25 μm capillary column (Agilent Technologies), at constant flow 1.5 ml.min-1 of helium as a carrier gas. One mi-croliter of derivatized sample was injected into the injector operating in 1:5 split mode. The temperature of the column was initially set to 60°C, and increased at a rate of 10°C.min−1 to 325°C. |
Instrument Name: | Agilent 7890B |
Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
Column Temperature: | 60 |
Flow Gradient: | N/A for GC |
Flow Rate: | 1.5 ml/min |
Solvent A: | N/A for GC |
Solvent B: | N/A for GC |
Chromatography Type: | GC |
Solvent C: | N/A for GC |