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MB Sample ID: SA301652

Local Sample ID:330P
Subject ID:SU002918
Subject Type:Cultured cells
Subject Species:Lentilactobacillus kefiri

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Combined analysis:

Analysis ID AN004571
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent HP5-MS (30m x 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977A
Ion Mode POSITIVE
Units normalized intensity

Chromatography:

Chromatography ID:CH003436
Chromatography Summary:The GC-MS analysis was carried out with an Agilent GC (Model 7890B) and MS Quadrupole (Model 5977A) (Agilent Technologies). The liquid injection was done using a PAL System RSI 85 (PAL, Lake Elmo, MN, USA). The injector temperature was 260°C; the MS transfer line was 230°C. Metabolites were separated on a 5% phenyl 95% dimethylarylene siloxane HP-5MS 30 m, 0.25 mm, 0.25 μm capillary column (Agilent Technologies), at constant flow 1.5 ml.min-1 of helium as a carrier gas. One mi-croliter of derivatized sample was injected into the injector operating in 1:5 split mode. The temperature of the column was initially set to 60°C, and increased at a rate of 10°C.min−1 to 325°C.
Instrument Name:Agilent 7890B
Column Name:Agilent HP5-MS (30m x 0.25mm, 0.25 um)
Column Temperature:60
Flow Gradient:N/A for GC
Flow Rate:1.5 ml/min
Solvent A:N/A for GC
Solvent B:N/A for GC
Chromatography Type:GC
Solvent C:N/A for GC
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