Return to study ST002813 main page
MB Sample ID: SA301807
| Local Sample ID: | Quad4 |
| Subject ID: | SU002921 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 14-15 weeks old |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004575 | AN004576 | AN004577 | AN004578 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity | Intensity | intensity |
Chromatography:
| Chromatography ID: | CH003439 |
| Chromatography Summary: | Chromatography for hydrophilic compounds |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters XBridge BEH Amide (150 x 2.1mm,2.5um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 min, 85% B; 2 min, 85% B; 3 min, 80% B; 5 min, 80% B; 6 min, 75% B; 7 min, 75% B; 8 min, 70% B; 9 min, 70% B; 10 min, 50% B; 12 min, 50% B; 13 min, 25% B; 16 min, 25% B; 18 min, 0% B; 23 min, 0% B; 24 min, 85% B; and 30 min, 85% B. |
| Flow Rate: | 150 uL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium acetate; mM ammonium hydroxide, pH 9.45) |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003440 |
| Chromatography Summary: | Chromatography for lipids |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C8 (150 x 2.1mm,2.7um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 minutes, 25% B; 2 minutes, 25% B; 4 minutes, 65% B; 16 minutes, 100% B; 20 minutes, 100% B; 21 minutes, 25% B; 27 minutes, 25% B |
| Flow Rate: | 150 uL/min |
| Solvent A: | 90% water/10% methanol; 1mM ammonium acetate ; 0.2% acetic acid |
| Solvent B: | 2% methanol/98% isopropano; 1 mM ammonium acetate; 0.2% acetic acid |
| Chromatography Type: | Reversed phase |
