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MB Sample ID: SA302637
| Local Sample ID: | T523 |
| Subject ID: | SU002926 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004584 | AN004585 | AN004586 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 | Waters Acquity |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Orbitrap |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak heights | peak heights | raw area counts rescaled to set the median equal to 1 |
Chromatography:
| Chromatography ID: | CH003445 |
| Chromatography Summary: | - |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | 0 min 15% (B), 0–2 min 30% (B), 2–2.5 min 48% (B), 2.5–11 min 82% (B), 11–11.5 min 99% (B), 11.5– 12 min 99% (B), 12–12.1 min 15% (B), 12.1–15 min 15% (B) |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60:40 v/v acetonitrile:water + 10 mM ammonium formate + 0.1% formic acid |
| Solvent B: | 90:10 v/v isopropanol:acetonitrile + 10 mM ammonium formate + 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003446 |
| Chromatography Summary: | Low pH polar (LC/MS Pos early) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
