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MB Sample ID: SA307223
Local Sample ID: | 90251016503 |
Subject ID: | SU002944 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004368 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Shimadzu Nexera X2 |
Column | Waters Atlantis HILIC (150 x 2.1 mm) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH003484 |
Chromatography Summary: | Extracts (10 µL) were injected onto a 150 x 2.1 mm Atlantis HILIC column (Waters). The column was eluted isocratically at a flow rate of 250 µL/min with 5% mobile phase A (10 mM ammonium formate and 0.1% formic acid in water) for 1 minute followed by a linear gradient to 40% mobile phase B (acetonitrile with 0.1% formic acid) over 10 minutes |
Methods Filename: | pass1b_hilicpos_methods.pdf |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Atlantis HILIC (150 x 2.1 mm) |
Flow Gradient: | 0-0.5 min, isocratic 95% B, 250 uL/min; 0.5-10.5 min, gradient to 40% B, 250 uL/min; 10.5-15 min, isocratic 40% B, 250 uL/min; 15-17 min, gradient to 95% B, 250 uL/min |
Flow Rate: | 0.25 ml/min |
Solvent A: | 100% water;10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |