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MB Sample ID: SA307244
| Local Sample ID: | SUB11589p2_Spl16 |
| Subject ID: | SU002945 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004631 | AN004632 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo BETASIL Diol (150 x 2.1mm,5um) | Thermo BETASIL Diol (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | area analyzed by Compound discoverer (counts x seconds) | area analyzed by Compound discoverer (counts x seconds) |
Chromatography:
| Chromatography ID: | CH003485 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo BETASIL Diol (150 x 2.1mm,5um) |
| Column Temperature: | 40 |
| Flow Gradient: | The LC program was as follow: starting at 93% B, to 40% B in 19 min, then to 0% B in 9 min, maintained at 0% B for 5 min, then back to 93% B in 3 min and re-equilibrated at 93% B for 9 min. |
| Flow Rate: | The flow rate was maintained at 0.15 mL/min, except for the first 30 seconds where the flow rate was uniformly ramped from 0.05 to 0.15 mL/ min. |
| Solvent A: | 20 mMAmmonium Carbonate, 0.1% Ammonium hydroxide, in Water |
| Solvent B: | Acetonitrile 97%, in water |
| Chromatography Type: | HILIC |
