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MB Sample ID: SA307517
| Local Sample ID: | Sd_Mn_Lys2 |
| Subject ID: | SU002951 |
| Subject Type: | Bacteria |
| Subject Species: | Bacillus subtilis |
| Taxonomy ID: | 1423 |
| Genotype Strain: | 168 |
| Age Or Age Range: | NA |
| Weight Or Weight Range: | NA |
| Height Or Height Range: | NA |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | NA |
| Cell Strain Details: | NA |
| Subject Comments: | NA |
| Cell Primary Immortalized: | NA |
| Cell Passage Number: | NA |
| Cell Counts: | NA |
| Species Group: | NA |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004649 | AN004650 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Normal phase | Normal phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Microsolv Diamond hydride (150 x 2.1mm,4um) | Microsolv Diamond hydride (150 x 2.1mm,4um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6220 TOF | Agilent 6220 TOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Area under the curve | Area under the curve |
Chromatography:
| Chromatography ID: | CH003499 |
| Chromatography Summary: | Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method. |
| Methods ID: | NA |
| Methods Filename: | NA |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Microsolv Diamond hydride (150 x 2.1mm,4um) |
| Column Pressure: | NA |
| Column Temperature: | 25 |
| Flow Gradient: | 0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B |
| Flow Rate: | 0.4 |
| Injection Temperature: | 25 |
| Solvent A: | Milli Q H2O + 0.2% Formic Acid |
| Solvent B: | Acetonitrile + 0.2% Formic Acid |
| Chromatography Type: | Normal phase |
| Solvent C: | NA |
| Chromatography ID: | CH003500 |
| Chromatography Summary: | Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Microsolv Diamond hydride (150 x 2.1mm,4um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B |
| Flow Rate: | 0.4 |
| Solvent A: | Milli Q H2O + 0.2% Formic Acid |
| Solvent B: | Acetonitrile + 0.2% Formic Acid |
| Chromatography Type: | Normal phase |
| Solvent C: | NA |
