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MB Sample ID: SA307543

Local Sample ID:c26_8
Subject ID:SU002952
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004651
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units Ion counts

Chromatography:

Chromatography ID:CH003501
Chromatography Summary:Lipidomics method
Instrument Name:Thermo Vanquish
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:55C
Flow Gradient:0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B
Flow Rate:0.2mL/min
Solvent A:90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Solvent B:75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase
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