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MB Sample ID: SA308642
| Local Sample ID: | tumor-enzalutamide-3-01 |
| Subject ID: | SU002964 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Genotype Strain: | NSG |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004672 | AN004673 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH003516 |
| Chromatography Summary: | Samples were run on a Vanquish (Thermo Fisher Scientific) UHPLC system with mobile phase A (20mM ammonium carbonate, pH 9.7) and mobile phase B (100% ACN) at a flow rate of 150 µL/min on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μm, EMD Millipore) at 35°C. Separation was achieved with a linear gradient from 20% A to 80% A in 20 minutes followed by a linear gradient from 80% A to 20% A from 20 minutes to 20.5 minutes. 20% A was then held from 20.5 minutes to 28 minutes. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
| Column Temperature: | 35°C |
| Flow Gradient: | Linear gradient was as follows: 20%A to 80%A (0-20 min), 80%A to 20%A (20-20.5 min), hold 20%A (20.5-28.0 min). |
| Flow Rate: | 150 µL/min |
| Solvent A: | 20 mM Ammonium carbonate, pH 9.7 |
| Solvent B: | 100% Acetonitrile |
| Chromatography Type: | HILIC |
