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MB Sample ID: SA308827
| Local Sample ID: | DS1-123-127 |
| Subject ID: | SU002969 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004679 | AN004680 | AN004681 | AN004682 | AN004683 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) | Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 120 | Thermo Orbitrap Exploris 120 | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area | Peak area | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003521 |
| Chromatography Summary: | Metabolomics Positive Mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0 min - 0.45 ml/min - 5% B, 0.5 min - 0.45ml/min - 5% B, 1.1 min - 0.45ml/min - 95% B, 2.75 min - 0.45ml/min - 95% B, 3 min - 0.45ml/min - 5% B, 5min - 0.45ml/min - 5%B |
| Flow Rate: | 0.45 ml/min |
| Solvent A: | 0.1% Formic Acid in Water |
| Solvent B: | 0.1% Formic Acid in ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003522 |
| Chromatography Summary: | Metabolomics Negative Mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0 min - 0.45 ml/min - 0% B, 0.5 min - 0.45ml/min - 0% B, 1.1 min - 0.45ml/min - 100% B, 2.75 min - 0.45ml/min - 100% B, 3 min - 0.45ml/min - 0% B, 5min - 0.45ml/min - 0%B |
| Flow Rate: | 0.45 ml/min |
| Solvent A: | 5% ACN 95% Water 1mM Ammonium Acetate |
| Solvent B: | 95% ACN 5% Water 1mM Ammonium Acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003523 |
| Chromatography Summary: | Lipidomics Negative |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min - 0.3ml/min - 10%B, 3 min - 0.3ml/min - 95%B, 4.2min - 0.3ml/min -95%B, 4.3min - 0.45ml/min - 10%B, 4.9min - 0.4ml/min - 10%B, 5 min - 0.3ml/min 10%B |
| Flow Rate: | 0.3-.45ml/min |
| Solvent A: | 75:25 H2O:ACN 5mM NH4OAc |
| Solvent B: | 90:10 iPrOH:ACN 5mM NH4OAc |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003524 |
| Chromatography Summary: | Lipidomics Positive |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min - 0.3ml/min - 30%B, 3 min - 0.3ml/min - 100%B, 4.2min - 0.3ml/min -100%B, 4.3min - 0.4ml/min - 30%B, 4.9min - 0/4ml/min - 30%B, 5 min - 0.3ml/min 30%B |
| Flow Rate: | 0.3-.4ml/min |
| Solvent A: | 75:25 H2O:ACN 5mM NH4OAc |
| Solvent B: | 90:10 iPrOH:ACN 5mM NH4OAc |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003525 |
| Chromatography Summary: | Oxylipin Method |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0min - 0.35ml/min - 0%B, 0.5min - 0.35ml/min - 25%B, 1min - 0.35ml/min - 25%B, 2.5min - 0.35ml/min 40%B, 2.6min -0.35ml/min 55%B, 4.5min - 0.35ml/min - 70%B, 4.6min - 0.35ml/min - 100%B, 6min - 0.35ml/min - 100%B, 6.1min - 0.35ml/min - 0%B, 7min - 0.35ml/min 0%B |
| Flow Rate: | .35ml/min |
| Solvent A: | 20:80:0.02 ACN:Water:Formic Acid |
| Solvent B: | 20:80:0.02 ACN:IPA:Formic Acid |
| Chromatography Type: | Reversed phase |
