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MB Sample ID: SA309466
| Local Sample ID: | NN-008 |
| Subject ID: | SU002976 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004696 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Phenomenex Luna NH2 (150 x 2mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Abundance |
Chromatography:
| Chromatography ID: | CH003536 |
| Chromatography Summary: | Dried metabolites were resuspended in 50% ACN:water and 1/10th was loaded onto a Luna 3um NH2 100A (150 × 2.0 mm) column (Phenomenex). The chromatographic separation was performed on a Vanquish Flex (Thermo Fisher Scientific) with mobile phases A (5 mM NH4AcO pH 9.9) and B (ACN) and a flow rate of 200 μl/minute. A linear gradient from 15% A to 95% A over 18 minutes was followed by 9 minutes isocratic flow at 95% A and reequilibration to 15% A. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Luna NH2 (150 x 2mm,3um) |
| Column Temperature: | 35 |
| Flow Gradient: | Linear gradient was as follows: 15% A to 95% A over 18 minutes was followed by 9 minutes isocratic flow at 95% A and reequilibration to 15% A |
| Flow Rate: | 200 ul/minute |
| Solvent A: | 5 mM NH4AcO pH 9.9 |
| Solvent B: | ACN |
| Chromatography Type: | HILIC |
