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MB Sample ID: SA314361
| Local Sample ID: | LN294 |
| Subject ID: | SU002987 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004711 |
|---|---|
| Analysis type | MS |
| Chromatography type | Normal phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH003547 |
| Chromatography Summary: | A volume of 5 μL of a standard or sample was injected onto an HSS T3 column, 2.1 × 100 mm, 1.8 μM column (Waters, Milford, MA, USA) at 30°C with a constant flow rate of 0.3 mL/min. Mobile phases consisted of (A) 0.1% formic acid in Milli-Q water and (B) 0.1% formic acid in ACN. The gradient program started at 99% A, decreased to 70% over 7.0 min, then decreased to 30% at 9 min, and return to the initial condition at 10 min and held for 4.0 min. The total runtime was 14 min. The weak and strong needles wash solutions were 5% ACN and H2O/ACN/MeOH/IPA (1:1:1:1), respectively. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | The gradient program started at 99% A, decreased to 70% over 7.0 min, then decreased to 30% at 9 min and return to the initial condition at 10 min and held for 4.0 min. The total runtime was 14 min. The weak and strong needles wash solutions were 5% ACN and H2O/ACN/MeOH/IPA (1:1:1:1), respectively. |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Normal phase |
