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MB Sample ID: SA315424

Local Sample ID:D-Glu/0_2-neg
Subject ID:SU003003
Subject Type:Bacteria
Subject Species:Escherichia coli
Genotype Strain:BL21(DE3)

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN004748 AN004749
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm. In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm.
MS Type ESI ESI
MS instrument type LTQ-FT LTQ-FT
MS instrument name Thermo Velos Pro Orbitrap Thermo Velos Pro Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Presence/Absence Presence/Absence

Chromatography:

Chromatography ID:CH003581
Chromatography Summary:Because the columns were packed in-house, there is some variation in the total length (see column description below).
Methods Filename:Deuterated_Lipids_Methods_Summary.pdf
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm.
Column Temperature:room temp
Flow Gradient:Solvent Gradient: Time (min) 0 1 2.5 7.5 13.5 48.5 58.5 75.5 77.5 87 Solvent A (%) 99 99 70 65 45 30 1 1 99 99 Solvent B (%) 1 1 30 45 55 70 99 99 1 1
Flow Rate:Split flow nano-chromatography. The actual flow rate at the column is dependent on a variety of factors and an accurate measurement of the flow rate is impractical. The flow rate at the macro pumps were set at either 0.05 or 0.1 mL/min depending on the waste line's backpressure.
Solvent A:60% acetonitrile/40% water; ~0.4g/L ammonium acetate
Solvent B:90% sopropanol/10% acetonitrile; ~0.4g/L ammonium acetate
Chromatography Type:Reversed phase
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