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MB Sample ID: SA315649
| Local Sample ID: | Alm 30.3 |
| Subject ID: | SU003015 |
| Subject Type: | Food item |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004761 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus BT |
| Column | Zebron ZB-FAME (30m × 0.25mm, 0.2um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 7890B |
| Ion Mode | POSITIVE |
| Units | uMol |
Chromatography:
| Chromatography ID: | CH003593 |
| Chromatography Summary: | The upper phase containing FAMEs was taken for analysis using a GC coupled to a time of flight mass spectrometry (GC-TOFMS, Pegasus BT, Leco Corp., St.Joseph, MI) on a ZB-FAME (30 m ×0.25 mm ×0.2 μm, Zebron) in EI mode. One microliter of sample was injected with a split ratio of 60 to 1. Helium was used as a carrier gas. The total flow was 1 mL min−1. The GC oven temperature was programmed from 40 °C (2 min hold) to 150 °C at 20 °C min−1 (1 min hold), to 165 °C at 1 °C min−1 (1 min hold), to 180 °C at 2 °C min−1, to 200 °C at 5 °C min−1, and to 260 °C at 40 °C min−1 (8 min hold). The transfer line and ion source temperatures were kept at 240 °C and 220 °C, respectively. The TOFMS scanned from m/z 45–650 with a data acquisition rate of 20 spectra s−1. MS data was acquired and processed using ChromaTOF software (version 5.32, Leco Corp.). |
| Instrument Name: | Leco Pegasus BT |
| Column Name: | Zebron ZB-FAME (30m × 0.25mm, 0.2um) |
| Column Temperature: | 40 |
| Flow Gradient: | na |
| Flow Rate: | 1 |
| Solvent A: | na |
| Solvent B: | na |
| Chromatography Type: | GC |
