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MB Sample ID: SA315696
Local Sample ID: | 7.3 |
Subject ID: | SU003015 |
Subject Type: | Food item |
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Combined analysis:
Analysis ID | AN004761 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus BT |
Column | Zebron ZB-FAME (30m × 0.25mm, 0.2um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7890B |
Ion Mode | POSITIVE |
Units | uMol |
Chromatography:
Chromatography ID: | CH003593 |
Chromatography Summary: | The upper phase containing FAMEs was taken for analysis using a GC coupled to a time of flight mass spectrometry (GC-TOFMS, Pegasus BT, Leco Corp., St.Joseph, MI) on a ZB-FAME (30 m ×0.25 mm ×0.2 μm, Zebron) in EI mode. One microliter of sample was injected with a split ratio of 60 to 1. Helium was used as a carrier gas. The total flow was 1 mL min−1. The GC oven temperature was programmed from 40 °C (2 min hold) to 150 °C at 20 °C min−1 (1 min hold), to 165 °C at 1 °C min−1 (1 min hold), to 180 °C at 2 °C min−1, to 200 °C at 5 °C min−1, and to 260 °C at 40 °C min−1 (8 min hold). The transfer line and ion source temperatures were kept at 240 °C and 220 °C, respectively. The TOFMS scanned from m/z 45–650 with a data acquisition rate of 20 spectra s−1. MS data was acquired and processed using ChromaTOF software (version 5.32, Leco Corp.). |
Instrument Name: | Leco Pegasus BT |
Column Name: | Zebron ZB-FAME (30m × 0.25mm, 0.2um) |
Column Temperature: | 40 |
Flow Gradient: | na |
Flow Rate: | 1 |
Solvent A: | na |
Solvent B: | na |
Chromatography Type: | GC |