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MB Sample ID: SA316312

Local Sample ID:1_UT5
Subject ID:SU003024
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Seven- to eight-week-old female BALB/c mice
Gender:Female
Animal Animal Supplier:Jackson Laboratories

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Combined analysis:

Analysis ID AN004780 AN004781
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity II Agilent 1290 Infinity II
Column Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6545 QTOF Agilent 6545 QTOF
Ion Mode POSITIVE NEGATIVE
Units AREA AREA

Chromatography:

Chromatography ID:CH003612
Instrument Name:Agilent 1290 Infinity II
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:50 °C
Flow Gradient:Started at 70% of B at 0 –1 min, 86% at 3.5 –10 min, and 100% B at 11–17 min
Flow Rate:0.6 mL/min
Solvent A:90% water/10% methanol; 10 mM ammonium acetate; 0.2 mM ammonium fluoride
Solvent B:20% acetonitrile/30% methanol/50% isopropanol; 10 mM ammonium acetate; 0.2 mM ammonium fluoride
Chromatography Type:Reversed phase
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