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MB Sample ID: SA323300

Local Sample ID:CSH_R4_Post_FMT_Pos
Subject ID:SU003090
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:61.1 +/- 15.2
Gender:Male and female
Human Lifestyle Factors:Unsuccessful antibiotic treatment for recurrent C.difficile infection
Human Medications:Vancomycin, Metronidazole, Fidaxomicin, probiotic

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Combined analysis:

Analysis ID AN004887 AN004888 AN004889 AN004890
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Orbitrap ID-X tribrid Thermo Orbitrap ID-X tribrid Thermo Orbitrap ID-X tribrid Thermo Orbitrap ID-X tribrid
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak area Peak area Peak area Peak area

Chromatography:

Chromatography ID:CH003688
Chromatography Summary:Individual subject samples, fractionated and unfractionated pooled samples, and analytical standards were analyzed by HILIC (Waters BEH Amide, 2.1 x 100 mm, 1.7 um) and RPLC at high pH (Waters Charged-Surface Hybrid [CSH] C18, 2.1 x 100 mm, 1.7 um) in both positive and negative ion modes on a Thermo Vanquish Horizon LC coupled to an Orbitrap ID-X mass spectrometer. For HILIC separations, mobile phase A consisted of 95:5 water:acetonitrile with 10 mM ammonium formate plus 0.125 % v/v formic acid and mobile phase B was 5:95 water:acetonitrile with the same additive concentrations. HILIC separations utilized the following gradient: 0 min, 100% B; 0-0.5 min 100% B; 0.5-7 min 85% B; 7-9 min 85% B; 9-16 min 50% B; 16-16.1 min 100% B; 16.1-20 min 100% B. For CSH separations, mobile phase A consisted of water with 10 mM ammonium acetate plus 0.025% ammonium hydroxide (v/v) and mobile phase B was methanol with the same additives
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:55
Flow Gradient:0 min, 100% B; 0-0.5 min 100% B; 0.5-7 min 85% B; 7-9 min 85% B; 9-16 min 50% B; 16-16.1 min 100% B; 16.1-20 min 100% B
Flow Rate:0.3 mL/min
Solvent A:95%water/5% acetonitrile; 10 mM ammonium formate; 0.125 % v/v formic acid
Solvent B:5% water/95% acetonitrile; 10 mM ammonium formate; 0.125 % v/v formic acid
Washing Buffer:9:1 water:methanol
Chromatography Type:HILIC
  
Chromatography ID:CH003689
Chromatography Summary:Individual subject samples, fractionated and unfractionated pooled samples, and analytical standards were analyzed by HILIC (Waters BEH Amide, 2.1 x 100 mm, 1.7 um) and RPLC at high pH (Waters Charged-Surface Hybrid [CSH] C18, 2.1 x 100 mm, 1.7 um) in both positive and negative ion modes on a Thermo Vanquish Horizon LC coupled to an Orbitrap ID-X mass spectrometer. For CSH separations, mobile phase A consisted of water with 10 mM ammonium acetate plus 0.025% ammonium hydroxide (v/v) and mobile phase B was methanol with the same additives. CSH separations utilized the following gradient: 0 min 0% B, 0-5 min 60% B; 5-13 min 99% B; 13-17 min 99% B; 17-17.1 min 0% B; 17-20 min 0% B.
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:55
Flow Gradient:0 min 0% B, 0-5 min 60% B; 5-13 min 99% B; 13-17 min 99% B; 17-17.1 min 0% B; 17-20 min 0% B
Flow Rate:0.45 mL/min
Solvent A:100% water; 10 mM ammonium acetate; 0.025% ammonium hydroxide (v/v)
Solvent B:100% methanol; 10 mM ammonium acetate; 0.025% ammonium hydroxide (v/v)
Washing Buffer:85:15 acetonitrile:water
Chromatography Type:Reversed phase
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