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MB Sample ID: SA326847

Local Sample ID:EF_eWAT_neg_4
Subject ID:SU003118
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:12 weeks
Weight Or Weight Range:25-30g
Gender:Male

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Combined analysis:

Analysis ID AN004935
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo orbitrap exploris 240
Ion Mode NEGATIVE
Units Peak area

Chromatography:

Chromatography ID:CH003724
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:25 °C
Flow Gradient:The linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min.
Flow Rate:0.4 mL/min
Solvent A:100% water; 25mM ammonium acetate; 25mM ammonium hydroxide
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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