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MB Sample ID: SA329863
| Local Sample ID: | NEG_10 |
| Subject ID: | SU003152 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 12 weeks |
| Weight Or Weight Range: | 25 g |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004984 | AN004985 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peakarea | peakarea |
Chromatography:
| Chromatography ID: | CH003765 |
| Chromatography Summary: | MSRC Lipidomics LC method |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | Linear gradient from 20% B to 100% B over 7 minutes |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM Ammonium formate, pH ~3 |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM Ammonium formate, pH ~3 |
| Chromatography Type: | Reversed phase |
