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MB Sample ID: SA333608
| Local Sample ID: | young_7 |
| Subject ID: | SU003219 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Young donors (aged 25 years or younger) and older donors (aged 50 years or older). |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN005082 | AN005083 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Waters Atlantis T3 (150 x 4.6mm,5um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| MS Type | API | API |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | abundance | abundance |
Chromatography:
| Chromatography ID: | CH003839 |
| Chromatography Summary: | Chromatographic method to detect analytes such as amino acids, endocannabinoids, fatty acid β-oxidation intermediates, gut-derived metabolites, nucleotides, neurotransmitters, cofactors and vitamins. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters Atlantis T3 (150 x 4.6mm,5um) |
| Column Temperature: | 40 |
| Flow Gradient: | The LC gradient program begins with an initial condition of 5% solvent A and 95% solvent B, with a flow rate of 0.25 mL/min, which is held for 0.5 minutes to establish system equilibration. The gradient then proceeds as follows: at 6 minutes, the mobile phase composition shifts to 60% solvent A and 40% solvent B for 3minutes; at 10 minutes, it changes back to 5% solvent A and 95% solvent B; at 11 minutes, the flow rate increases to 0.4 mL/min while maintaining a composition of 5% solvent A and 95% solvent B for a duration of 12.5 minutes; and at 24.5 minutes, the flow rate decreases back to 0.25 mL/min. The final condition is maintained for 1 minutes to ensure stability before subsequent analyses. |
| Flow Rate: | 0.250 – 0.400 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate (pH ~2.5) |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003840 |
| Chromatography Summary: | Chromatographic method to detect analytes including amino acids, nucleotides, nucleosides, nucleotide triphosphates, high energy intermediates, organic acids, TCA cycle intermediates, bile acids and vitamins. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| Column Temperature: | 40 |
| Flow Gradient: | The LC gradient program is initialized with an initial mobile phase composition of 15% solvent A and 85% solvent B at a flow rate of 0.25 mL/min. Over the course of 8 minutes, the mobile phase composition undergoes a transition to 65% solvent A and 35% solvent B. Subsequently, at 8 minutes, the composition shifts to 98% solvent A and 2% solvent B, maintained for 1 minute. The mobile phase reverts back to the initial composition of 15% solvent A and 85% solvent B at 10 minutes. At 12.5 minutes, the flow rate is increased to 0.5 mL/min, while maintaining a constant mobile phase composition of 15% solvent A and 85% solvent B for a period of 2.5 minutes. Finally, at 15 minutes, the flow rate is reduced back to 0.25 mL/min. To ensure system stability, the final condition is maintained for 1 minute prior to subsequent analyses. |
| Flow Rate: | 0.250 – 0.400 mL/min |
| Solvent A: | 95% acetonitrile/5%; water 20mM ammonium acetate; 20mM ammonium hydroxide (pH 9.0) |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
