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MB Sample ID: SA333654

Local Sample ID:SUB13607p4_SPL08
Subject ID:SU003221
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN005085
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap ID-X tribrid
Ion Mode UNSPECIFIED
Units area analyzed by Compound discoverer (counts x seconds)

Chromatography:

Chromatography ID:CH003841
Instrument Name:Thermo Vanquish
Column Name:Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um)
Column Temperature:40
Flow Gradient:Started at 93% B and 7% A ; 40% B/60% A in 19 min; 100% A in 9 min; 100% A for 5 min; back to 93% B/7% A in 3 min; re-equilibration at 93% B/7% A for 9 min.
Flow Rate:0.05 to 0.15 mL/min in 30 sec; then held at 0.15mL/min
Solvent A:100% Water; 20 mMAmmonium Carbonate, 0.1% Ammonium hydroxide
Solvent B:Acetonitrile 97%, 3% water
Chromatography Type:HILIC
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