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MB Sample ID: SA337394
| Local Sample ID: | S12_mitos_control_4 |
| Subject ID: | SU003230 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005102 | AN005103 | AN005104 | AN005105 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | None (Direct infusion) | Normal phase | Reversed phase | Reversed phase |
| Chromatography system | Advion TriVersa NanoMate | Agilent 1260 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | None | Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) | Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) | Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTRAP | QTRAP | QTRAP | QTRAP |
| MS instrument name | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE |
| Units | counts per second (cps) | counts per second (cps) | counts per second (cps) | counts per second (cps) |
Chromatography:
| Chromatography ID: | CH003858 |
| Chromatography Summary: | Lipid extract infusion and ionization was conducted using Nano-ESI chips with the TriVersa NanoMate operated by the ChipSoft Software (Advion) under the following settings: sample infusion volume: 14 μl, volume of air to aspirate after sample: 1 μl, air gap before chip: enabled, aspiration delay: 0 s, prepiercing: with mandrel, spray sensing: enabled, cooling temperature: 14°C, gas pressure: 0.5 psi, ionization voltage: 1.4 kV, and vent headspace: enabled. Prewetting was done once. |
| Instrument Name: | Advion TriVersa NanoMate |
| Column Name: | None |
| Column Temperature: | N/A |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | None |
| Solvent B: | None |
| Chromatography Type: | None (Direct infusion) |
| Chromatography ID: | CH003859 |
| Chromatography Summary: | Note: Macherey-Nagel does not offer this column as standard, but manufactures it on request. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) |
| Column Temperature: | 20 |
| Flow Gradient: | 0 min: 0% B, 0.5 min: 0% B, 0.7 min: 10% B, 1.2 min: 10% B, 1.6 min: 18% B, 2.2 min: 18% B, 2.6 min: 100% B, 4.5 min: 100% B, 4.9 min: 0% B, 6.5 min: 0% B |
| Flow Rate: | 0.75 ml/min |
| Solvent A: | 97% acetonitrile/2% methanol/1% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 99% methanol/1% acetic acid; 5 mM ammonium acetate |
| Chromatography Type: | Normal phase |
| Chromatography ID: | CH003860 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min: 30% B, 0.5 min: 30% B, 4.5 min: 68% B, 20.5 min: 75% B, 21 min: 97% B, 24 min: 97% B, 24.5 min: 30% B, 28 min: 30% B |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003861 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min: 55% B, 4 min: 95% B, 7 min: 95% B, 7.1 min: 55% B, 10 min: 55% B |
| Flow Rate: | 0.7 ml/min |
| Solvent A: | 100% water; 0.012 % acetic acid; 5 mM ammonium acetate |
| Solvent B: | 80% acetonitrile/20% isopropanol |
| Chromatography Type: | Reversed phase |
