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MB Sample ID: SA338969
| Local Sample ID: | SL_HetCD_3 |
| Subject ID: | SU003245 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005128 | AN005129 | AN005130 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm, 5um) | Imtakt Cadenza CD-C18 (150 x 2.1 mm, 3um) | Imtakt Cadenza CD-C18 (150 x 2.1 mm, 3um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED | NEGATIVE | POSITIVE |
| Units | Peak Intensity | Peak Intensity | Peak Intensity |
Chromatography:
| Chromatography ID: | CH003880 |
| Chromatography Summary: | Serum targeted and untargeted metabolomics |
| Methods Filename: | Serum_MS_and_Data_Analysis.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm, 5um) |
| Column Temperature: | 35 |
| Flow Gradient: | 85% to 30% A in 20 min followed by a wash with 30% A and re-equilibration at 85% A |
| Flow Rate: | 150 µl/min |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water; 0.1% ammonium hydroxide; 20 mM ammonium acetate |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003881 |
| Chromatography Summary: | Serum free fatty acids |
| Methods Filename: | Serum_MS_and_Data_Analysis.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Imtakt Cadenza CD-C18 (150 x 2.1 mm, 3um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0–1.5 min, 50% B; 1.5-3 min, 50-90% B; 3-5.5 min, 90-95% B; 5.5-10 min, 95% B, followed by 5 min of re-equilibration |
| Flow Rate: | 150 µl/min |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 85% isopropanol/10% acetonitrile/5% water; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003882 |
| Chromatography Summary: | Serum lipidomics |
| Methods Filename: | Serum_MS_and_Data_Analysis.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Imtakt Cadenza CD-C18 (150 x 2.1 mm, 3um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0–1.5 min, 32% B; 1.5-4 min, 32-45% B; 4-5 min, 45-52% B; 5-8 min, 52-58% B; 8-11 min, 58-66% B; 11-14 min, 66-70% B; 14-18 min, 70-75% B; 21-25 min, isocratic 97% B, 25-25.1 min 97-32% B |
| Flow Rate: | 200 µl/min |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
