Summary of Study ST000165
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000143. The data can be accessed directly via it's Project DOI: 10.21228/M8GP4N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Download additional data: the amino acids were measured under negative ion chemical ionisation conditions using isobutane as reactant gas
| Study ID | ST000165 |
| Study Title | Sparing of muscle mass and function by passive loading in an experimental intensive care unit model |
| Study Type | time course + intervention |
| Study Summary | A unique experimental rat ICU model has been used allowing long-term (weeks) time-resolved analyses of the effects of standardized unilateral passive mechanical loading on skeletal muscle size and function and underlying mechanisms. Results show that passive mechanical loading alleviated the muscle wasting and the loss of force-generation associated with the ICU intervention, resulting in a doubling of the functional capacity of the loaded versus the unloaded muscles after a 2-week ICU intervention. |
| Institute | Uppsala University |
| Department | Department of Neuroscience |
| Last Name | Larsson |
| First Name | Lars |
| Lars.larsson@neuro.uu.se | |
| Submit Date | 2015-05-14 |
| Num Groups | 2 |
| Total Subjects | 13 |
| Raw Data Available | No |
| Analysis Type Detail | IR-MS |
| Release Date | 2015-05-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000258 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | |
| Column | |
| MS Type | IR MS |
| MS instrument type | IR MS |
| MS instrument name | Thermo DeltaPlus Isotope Ratio MS |
| Ion Mode | NEGATIVE |
| Units |
Chromatography:
| Chromatography ID: | CH000182 |
| Chromatography Summary: | The level of enrichment of [ring-13C6]phenylalanine derived from hydrolysed mixed muscle proteins were analysed using a ThermoFisher DeltaPlus Isotope Ratio mass spectrometer (IR/MS) (Bremen, Germany) fitted with an on-line gas chromatograph with oxidation and reduction furnaces as previously described (Balagopal et al. 1996). The amino acids were derivatized to their trimethyl acetyl, methyl esters according to the method of Metges et al. (1996). Any amino acid eluting from the gas chromatograph is converted to CO2 and N2 prior to entry into the IR/MS. The amino acids were derivatized to their trimethyl acetyl, methyl esters according to the method of Metges et al. (1996). Enrichment of the tracer was measured by monitoring the ratio of 13C to 12CO2 in the IR/MS and again referenced to a calibration curve (00.1%). |
| Chromatography Type: | GC |
